Addgene - EGFP-LC3 Plasmid Data

Gene/insert name:		EGFP-LC3B
Alternative names:		NM_022818
		Human homolog of yeast ATG8p
Insert size (bp):		380
GenBank/Entrez ID of insert:		NM_022818
Gene/insert aliases:		MAP1LC3B, MAP1A/1BLC3
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		EGFP
Terminal:		N terminal on insert
Vector backbone:		pEGFP-C3 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		4727
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		CMV Forward (List of Sequencing Primers)
3' Sequencing primer:		EBV Reverse
Bacteria resistance:		Kanamycin
High or low copy:		Don't Know
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		Kopito Lab - see reference
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Karla Kirkegaard
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

65 - 352

CMV_immearly_promoter

10 - 562

CMV_fwd_primer

519 - 539

CMV_promoter

520 - 589

EGFP_N_primer

679 - 658

EGFP

613 - 1329

Orf frame 1

613 - 1740

EGFP_C_primer

1266 - 1287

SV40_PA_terminator

1794 - 2022

EBV_rev_primer

1992 - 2011

f1_origin

2467 - 2161

AmpR_promoter

2546 - 2574

pBABE_3_primer

2660 - 2640

SV40_enhancer

2861 - 2646

SV40_promoter

2658 - 2926

SV40_origin

2825 - 2902

SV40pro_F_primer

2887 - 2906

Orf frame 3

3009 - 3803

Neomycin

3012 - 3800

TK_PA_terminator

3978 - 4247

pBR322_origin

4395 - 5014

Unique restriction sites

AseI		7
NdeI		234
NheI		591
AgeI		600
BglII		1335
XhoI		1339
HindIII		1348
PstI		1750
SalI		1751
KpnI		1761
SacII		1764
ApaI		1769
SmaI		1770
BamHI		1772
XbaI		1784
BclI		1794
HpaI		1900
ClaI		2977
EagI		3043
NarI		3137
MscI		3219
FspI		3239
ApaLI		4741

Article: Subversion of cellular autophagosomal machinery by RNA viruses. Jackson WT et al. (PLoS Biol. 2005 May . 3(5):e156. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11546" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.