• Gene/insert name:

  PINK1

• Insert size:

  1743

• Species:

  H. sapiens (human)

• Entrez Gene:

  PINK1 (BRPK, PARK6, FLJ27236)
  

• Fusion protein or tag:

  myc

• Terminal:

  C terminal on backbone

• Vector backbone:

  pCMVTNT
  (Search Vector Database)

• Backbone manufacturer:

  Promega

• Vector type:

  Mammalian Expression

• Backbone size w/o insert:

  4050

• Cloning site 5':

  EcoRI

• Site destroyed during cloning:

  No

• Cloning site 3':

  NotI

• Site destroyed during cloning:

  No

• 5' sequencing primer:

  T7 List of Sequencing Primers

• 3' sequencing primer:

  EBV-rev

• Bacterial resistance(s)

  Ampicillin

• Growth strain(s)

  DH5alpha

• Growth temperature (℃):

  37

• High or low copy:

  High Copy

• Sequence: View sequences (2)
• Supplemental document:

  Notes from Addgene (1)

• Principal Investigator:

  Mark Cookson

• Terms and Licenses:

  MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mutations in PTEN-induced putative kinase 1 associated with recessive parkinsonism have differential effects on protein stability. Beilina et al (Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13314" in your Materials and Methods section.