• Gene/insert name:

  LDLR promoter mut SRE

• Alt name:

  LDL receptor promoter

• Alt name:

  LDL receptor

• Insert size:

  340

• Species:

  H. sapiens (human)

• GenBank ID:

  NC_000019

• Entrez Gene:

  LDLR (FH, FHC, LDLCQ2)
  

• Fusion protein or tag:

  luciferase

• Terminal:

  C terminal on backbone

• Mutation:

  Point mutation in the SRE-1 sequence of the LDL receptor promoter (ATCACCCCAC changed to ATAACCCCAC)

• Vector backbone:

  pGL2 basic
  (Search Vector Database)

• Backbone manufacturer:

  Promega

• Vector type:

  Mammalian Expression, Luciferase

• Backbone size w/o insert:

  5597

• Cloning site 5':

  SacI

• Site destroyed during cloning:

  No

• Cloning site 3':

  XhoI

• Site destroyed during cloning:

  No

• 5' sequencing primer:

  n/a List of Sequencing Primers

• 3' sequencing primer:

  LucNrev

• Bacterial resistance(s)

  Ampicillin

• Growth strain(s)

  DH5alpha

• Growth temperature (℃):

  37

• High or low copy:

  High Copy

• Sequence: View sequences (2)
• Map:

  View map

• Principal Investigator:

  Axel Nohturfft

• Terms and Licenses:

  MTA
  Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Castoreno et al (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14945" in your Materials and Methods section.