Addgene - pCMV Fcgamma RIIa IRES neo Plasmid Data

Gene/insert name:
Fcgamma RIIa
Alt name:
FcgRIIa
Alt name:
Fc gamma RIIa
Insert size:
1000
Species:
H. sapiens (human)
Entrez Gene:
FCGR2A (CD32, FCG2, FcGR, CD32A, CDw32, FCGR2, IGFR2, FCGR2A1, MGC23887, MGC30032)
Vector backbone:
pIRESneo2
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert:
5300
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance:
Ampicillin
Growth strain:
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Axel Nohturfft
Terms and Licenses:
MTA

Comments:

The FcgRIIa cDNA was amplified by RT-PCR using a Titan One Tube RT-PCR System kit (Roche, Mannheim, Germany) and a pair of primers (5'-TTGAATTCACCATGGAGACCCAAATGTCTC-3' and 5'-GCGGCCGCTTAGTTATTACTGTTGACATGG-3'). The PCR product was cloned into pCR2.1-TOPO (Invitrogen, Carlsbad, CA) to generate pAC01 and subcloned as an EcoRI–NotI fragment into pIRESneo2 (Clontech, Mountain View, CA) to generate pCMV-FcgRIIa-IRES-Neo.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Immunoglobulin G signaling activates lysosome/phagosome docking. Trivedi et al (Proc Natl Acad Sci U S A. 2006 Nov 28. 103(48):18226-31. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14948" in your Materials and Methods section.