Addgene - pLOVE-GFP Plasmid Data

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Miguel Ramalho-Santos Lab Plasmids

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Plasmid 15949: pLOVE-GFP

Gene/insert name:		Green Fluorescent Protein
Alternative names:		EGFP
Insert size (bp):		600
Species of gene(s):		Aequorea victoria
Relevant mutations/deletions:		The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-GFP is ~7407 bp.
Fusion proteins or tags:		V5
Terminal:		C terminal on insert
Vector backbone:		pLOVE (Search Vector Database)
Type of vector:		Lentiviral
Backbone size (bp):		8263
5' Sequencing primer:		n/a (List of Sequencing Primers)
3' Sequencing primer:		V5
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Please specify bacterial strain for growth and growth condition:		Expand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
Sequence:		View sequence
Plasmid Provided In:		Stbl3
Principal Investigator:		Miguel Ramalho-Santos
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R et al. (Cell Stem Cell. 2007 Sep 13. ():.)

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