Addgene - pBABE-puro Plasmid Data

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Notes

Related Plasmids

From this article

Bob Weinberg Lab Plasmids

This is commonly
requested with

pBABE-neo

pBABE-hygro

pBABE GFP

pBABE-zeo ( pBABE-bleo)

pCMV-VSV-G

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Plasmid 1764: pBABE-puro

Gene/insert name:		none
Insert size (bp):		Unknown
Vector backbone:		pBABE-puro (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral
Backbone size (bp):		5169
5' Sequencing primer:		pBABE 5' (List of Sequencing Primers)
3' Sequencing primer:		pBABE 3'
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Puromycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Bob Weinberg
Additional Documents:		pBABE protocol (PDF)
Terms and Licenses:		MTA

Comments: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that Addgene supplies pCL-Eco (#12371), VSV-G (#8454), and a gag/pol expression vector (#8455).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

5_LTR2

12 - 479

3MoMuLV_LTR

18 - 479

psi_plus_pack

549 - 1350

pBABE_5_primer

1318 - 1334

pBABE_3_primer

1428 - 1408

SV40_enhancer

1629 - 1414

SV40_promoter

1426 - 1694

SV40_origin

1593 - 1670

SV40_promoter

1546 - 1748

SV40pro_F_primer

1655 - 1674

Orf frame 3

1752 - 2351

puro(0-600)

1752 - 2351

3MoMuLV_LTR

2450 - 3043

5_LTR2

2492 - 3043

pBR322_origin

4019 - 3400

Ampicillin

5041 - 4181

AmpR_promoter

5111 - 5083

Unique restriction sites

SpeI		616
BamHI		1355
EcoRI		1379
SalI		1397
NcoI		1634
HindIII		1743
SacII		1966
ClaI		2406
NheI		2480
NotI		4133
FspI		4476

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1764" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.