Addgene - Flag-SIRT1 Plasmid Data

Gene/insert name:		SIRT1
Alternative names:		Sir2
Insert size (bp):		Unknown
Gene/insert aliases:		SIRT1, SIR2L1
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		Flag
Terminal:		N terminal on insert
Vector backbone:		pECE (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		2900
Cloning site 5':		HindIII
Site destroyed during cloning:		No
Cloning site 3':		XbaI
Site destroyed during cloning:		No
5' Sequencing primer:		SV40pro-F (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Michael Greenberg
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Article: Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Brunet A et al. (Science 2004 Mar 26;303(5666):2011-5. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1791" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.