Special Collections > TALEN Kits > TALEN Kit #1000000016

Golden Gate TALEN and TAL Effector Kit

See the TALe feature article in the December 2011 Addgene Newsletter

TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. This collection and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 of these repeats. The reagents include a plasmid construct for making custom TAL effectors and one for TAL effector fusions to additional proteins of interest. The web based software which allows one to search for TAL binding sites is located at:

http://boglabx.plp.iastate.edu/TALENT/

This set of plasmids is described in

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. PUBMED. Open Access Article.

Download a PDF Protocol (Included with Shipment)

Download a ZIP file containing all plasmid sequences (except pKEB31) in GenBank format and all plasmid maps in JPEG format

Purchase the Golden Gate TALEN and TAL Effector Kit (cat#1000000016)

The kit is $350, plus shipping and handling. This kit will be shipped to most countries as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80oC immediately upon arrival. Scientists in countries that cannot receive dry ice shipments (see FAQ page) will receive a room temperature version of the kit that needs to be processed immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

The TALEN plasmids fill 72 wells on a single 96-well plate.

Note that Addgene uses UPS for shipments and can ship dry ice to most countries, excluding China. Scientists from China interested in obtaining this kit should contact help@addgene.org.