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DECIPHER Project

DECIPHER libraries are bar-coded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover the entire annotated human and mouse gene sets. Lentiviral shRNA libraries were chosen over synthetic siRNA collections for their advantages in cancer research, specifically for the ability to efficiently transduce a range of cell types, including non-dividing cells, and for the highly effective long-term gene silencing. The pooled format screens follow a simple protocol and do not require access to expensive high-throughput arrayed screen equipment and infrastructure, putting the genome-wide shRNA knockdown screens within reach of any researcher.

Designed Specifically for Screens in Pooled Format Several DECIPHER library design features make them considerably more effective than other currently available RNAi libraries. The high percentage of functionally validated sequences and inclusion of a redundant set of 5 to 6 shRNA per gene results in at least 70% knockdown efficiency for approximately 65% of the target genes represented (depending on the cell type). The vast majority of shRNA constructs in the library are represented equally, with the difference in concentrations not exceeding one order of magnitude. Typically, about 90% of the population of shRNA constructs are present within a 10-fold range.

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