Showing: 1 - 20 of 26539 results
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Plasmid#85126PurposeNSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.DepositorInsertPkaiB-luciferase
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAM4731
Plasmid#85127PurposeNSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.DepositorInsertPkaiBC-KaiB
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
UseTagsAmilCP and RTXExpressionBacterialMutationPromoterJ23100 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI Methylase-AmilCP
Plasmid#85166PurposeFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.DepositorInsertEcoRI Methylase-AmilCP
UseTagsAmilCPExpressionBacterialMutationPromoterJ23100 promoterAvailabilityAcademic Institutions and Nonprofits only -
pBBR1MCS-2
Plasmid#85168PurposeMobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsExpressionBacterialMutationPromoterConstitutive (derepressed P-lac)AvailabilityIndustry, Academic Institutions, and Nonprofits -
RGD-ACP-I27-CC
Plasmid#85391PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and ACP tag for fluorophore labelingDepositorInsertRGD motif for integrin binding fused to acyl carrier protein (ACP) tag and titin immunoglobulin domain (I27)
UseTags6x HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
RGD-sfGFP-I27-CC
Plasmid#85392PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labelingDepositorInsertRGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
UseTags6x HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
RGD-I27-CC
Plasmid#85393PurposeMTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertRGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10)-I27-CC
Plasmid#85394PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10, RGE)-I27-CC
Plasmid#85396PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationGRGDS motif mutated to GRGESPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi
Plasmid#85455PurposemWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
UseTags6xHisExpressionBacterialMutationD341APromoterTlpA and TlpA, LacIAvailabilityAcademic Institutions and Nonprofits only -
pLPT20
Plasmid#85460PurposeRepressilator with integrated reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
UseSynthetic BiologyTagsssrAExpressionBacterialMutationPromoterPLlac, PLtet, PR, and PRNAIAvailabilityAcademic Institutions and Nonprofits only -
pGST-PSP-hTG
Plasmid#85470Purposebacterial expression of human tissue transglutaminaseDepositorInserthTG2 (TGM2 Human)
UseTagsGST and PSPExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pRSETB-de4GFP
Plasmid#85472PurposeInducible expression of dual emitting pH sensitive GFP in bacterial cellsDepositorInsertde4GFP
UseTags6xHisExpressionBacterialMutationS65T/C48S/H148C/T203CPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterArabinoseAvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP 150TAG
Plasmid#85483PurposeArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150TAG (amino acid 150 mutated to stop codon)PromoterArabinoseAvailabilityAcademic Institutions and Nonprofits only -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
UseTagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 26539 results