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Addgene

MAC-C Sars-CoV-2 S_Y144del
(Plasmid #194854)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 194854 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    MAC-tag-C (#108077)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S_Y144del
  • Alt name
    Spike protein Y144 deletion
  • Species
    Sars-CoV-2
  • Mutation
    Y144del
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • MAC-C (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC-C Sars-CoV-2 S_Y144del was a gift from Markku Varjosalo (Addgene plasmid # 194854 ; http://n2t.net/addgene:194854 ; RRID:Addgene_194854)
  • For your References section:

    The comprehensive SARS-CoV-2 ‘hijackome’ knowledge base— reveals significant changes in host cell protein expression and activation by multiple SARS-CoV-2 variants. Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P, Belanov S, Auvinen P, Vartiainen M, Laine L, Österlund P, Julkkunen I, Kakkola L. 10 May 2024, Research Square 10.21203/rs.3.rs-4322477/v1